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| TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan. |
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Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582 |
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| MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi. |
| We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity... |
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Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581 |
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| 猪熊, 壽; 滄木, 孝弘; 山川, 和宏; 竹内, 俊彦; 松本, 高太郎; 石井, 三都夫; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; YAMAKAWA, Kazuhiro; TAKEUCHI, Toshihiko; MATSUMOTO, Kotaro; ISHII, Mitsuo; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi. |
| 病理学的に血様液貯留を特徴とする特発性心嚢水腫と診断された乳牛8症例（血様心嚢水貯留群）の臨床病理学的検査所見を回顧的に取りまとめ，心膜炎7症例（心膜炎群）の所見と比較した．血様心嚢水貯留群では冷性浮腫を呈する症例が心膜炎群に比べて多かったが，その他の臨床症状には両群で差がなかった．心エコー検査では血様心嚢水貯留群では，心膜炎でみられる心嚢内の顕著なフィブリン析出はなかった．血液及び生化学検査では，血様心嚢水貯留群では炎症像が弱く，心膜炎群と比較して白血球数，分葉核好中球割合，A/G比，アルブミン濃度，γグロブリン割合に有意差がみられた．両群でALP及びGGTの高値がみられたが，特に血様心嚢水貯留群のGGT増加は顕著であった．血様心嚢水貯留症例の診断は臨床症状だけでは困難であるが，心エコー検査所見，弱い炎症像，ALP及びGGTの高値などが心膜炎との鑑別診断に有用と考えられた． Clinicopathological findings of eight cases of idiopathic hydropericardium characterized by bloodhlike fluid (HBFhgroup) that were confirmed by necropsy were retrospectively summarized and compared with those of seven cows with pericarditis. Although the numbers of cows that showed edema in the HBFhgroup were larger than those in the pericarditishgroup, the frequency of... |
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Palavras-chave: 特発性心餒水腫; 心膜炎; 血様心嚢水; 牛; Idiopathic hydropericardium; Pericarditis; Blood-like pericardium; Cow. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3557 |
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| 竹内, 俊彦; 吉本, 薫; 駒形, 真; 福中, 守人; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; TAKEUCHI, Toshihiko; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; FUKUNAKA, Morito; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi. |
| 5歳5カ月齢のホルスタイン種雌牛が，発熱，食欲不振及び胎盤停滞を呈した．悪露排出及び子宮の腫脹は治療で改善がみられなかった．体表リンパ節の腫大はみられなかったが，直腸検査で子宮の硬結を認めたため，牛白血病を疑った．病理解剖では腹腔内リンパ節の腫大，胃漿膜面の腫瘤形成及び子宮壁の肥厚が認められた．また，組織所見ではこれら組織へのリンパ球様腫瘍細胞の浸潤が認められた．腫瘍細胞はB細胞マーカー陽性であった．牛白血病ウイルス（BLV）抗体は陰性であったが，末梢血のBLVプロウイルス検査では陽性が確認され，本症例におけるBLVの関与が示唆された． A 5-year-5-month old Holstein cow was presented with fever, anorexia, and retained placenta. Postnatal vaginal discharge and enlargement of the uterus did not improve after treatment. Although the superficial lymph nodes were not enlarged, enzootic bovine leukosis was suspected because of the enlarged and indurated uterus. An autopsy revealed enlargement of most abdominal lymph nodes, tumor mass formation on the omasum and abomasum, and thickening of the uterine wall. Histologically, there... |
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Palavras-chave: 牛白血病ウイルス; 慢性子宮内膜炎; 地方病性牛白血病; Bovine leukosis virus; Chronic endometritis; Enzootic bovine leukosis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3555 |
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